CLICK HERE FOR BLOGGER TEMPLATES AND MYSPACE LAYOUTS »

Thursday, October 22, 2009

=.i hate this 20 hours!.=

it was just totally nightmare for me.
7pm, skipped my lunch and dinner, by only having 180kcal energy bar.
7.45pm, going for kid's tuition until 9.30pm.
10pm, started to get into with my planning for cell harvest protocol.
12am, end my planning and started to packed, ready to go to bed.
12.10am, stomach start to grumble on me, forcing me rush into toilet.
12.20am, second time.
12.40am, third time.
1.15am, fourth time...certified - diarrhoea.
1.30am, it's better and i fall asleep after all.
5.30am, AGAIN.
5.40am, cant stand the hard feeling, apply chinese herbs medicine.
5.50am, slowly fall asleep again.
6.30am, alarm clock rang, time to go for my lab again.
8.30am, start to capturing almost 50 photos for my raji cells.
9.10am, transfering the cells into eppendof tubes, one by one.
9.45am, start to cell counting, 21 x 4 = 84 blocks to count~!
12.25pm, start to spin down my cells for 10 minutes.
12.35pm, remove supernatant, adding PBS, resuspend..x3
2pm, rushing to alpha angle for some emergency meetup.
3pm, continue with the teflon-slide preparation.
4.30pm, waiting for the PBS in the slides to DRY NATURALLY.
4.45pm, cell culture in big flasks.
5pm, preparation for the FITC sub-stock.
5.20pm, damn slides still NOT YET DRY.
5.45pm, finally it looks dry...fixed with ice-cold acetone, 10 mins.
5.55pm, finally i get done with my job. clean up everything.
6.10pm, fetch kitson to LRT station.
6.30pm, badly jam, finally reached genting klang.
6.50pm, finally, my dinner after a cup of coffee this morning.

-xoxo-

0 comments: